Derivative Spectroscopy: Development and Validation of New Spectroscopic Method for The Estimation of Metadoxine in Bulk and Solid Dosage Form

P. Kumar¹, D.S. Mittan³, A. Malik², N. Kaushik², A. Kushnoor² and N.

¹Department of Pharmaceutical Analysis, PES college of Pharmacy, Bangalore India.

²Shri Gopichand College of Pharmacy, Baghpat India.

³Pharmacy Department, Faculty of Technology and Engineering, The M. S. University of Baroda India.

ABSTRACT:

A simple and sensitive spectroscopic method in ultraviolet region was developed and validated for the estimation of Metadoxine in pure and pharmaceutical dosage forms by derivative spectroscopy. The method is based on Metadoxine, showing absorbance at 292, 302, 270 and 314 nm for zero order, first order, second order and third order derivative spectroscopy respectively in distilled water. But regression values with best results were found to be best for third order derivative spectroscopy. The method obeys Beers law in the concentration range of 4 to 40μg/ml. The proposed method is precise, accurate, linear, stable and reproducible and can be extended to the analysis of Metadoxine in bulk and tablet formulations.

KEYWORDS:

Derivative spectroscopy; Metadoxine; UV spectroscopic; U.V estimation

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Copy the following to cite this article: Kumar P, Mittan D. S, MMalik A, Kaushik N, Kushnoor A, and N.. Derivative Spectroscopy: Development and Validation of New Spectroscopic Method for The Estimation of Metadoxine in Bulk and Solid Dosage Form. Orient J Chem 2008;24(1).

Copy the following to cite this URL: Kumar P, Mittan D. S, MMalik A, Kaushik N, Kushnoor A, and N.. Derivative Spectroscopy: Development and Validation of New Spectroscopic Method for The Estimation of Metadoxine in Bulk and Solid Dosage Form. Orient J Chem 2008;24(1). Available from: http://www.orientjchem.org/?p=23317