Analysis of Haemoglobin Variants by Cation Exchange HPLC (High Performance Liquid Chromatography)

C. B. S. Dangi*, N. C. Sharma and S. Purey

Department of Human Genetics, Barkatullah University, Bhopal - 462 026 (India)

Analysis of Haemoglobin Variants in present study was identified by cation exchange HPLC among 50 clinically suspected cases of haemoglobinopathies in population of Bhopal. Out of the 50 cases of haemoglobin disorders, the largest group was formed by variant of beta-thalassaemia (58%), out of which beta-thalasaemia minor constituted 22% of the patients and beta-thalassaemia intermedia and major constituted 18% each. The Sickling disorders formed the second largest group (38%) with Sickle beta-thalassaemia and Sickle cell anaemia comprising 22% and 16% respectively. In variant of Sickle beta-thalassaemia, seven cases had Sickle beta+ thalassaemia and four cases had Sickle beta0 thalassaemia. Haemoglobin variant of E beta-thalassaaemia formed the remaining 4% of the cases. One patient of Thalassaemia minor also showed an unknown peak of 18.6% in the HPLC system at a retention time of 1.93 mins. On screening, his father was also found to have a peak of 20.6% appearing at the same retention time.

HPLC (High performance liquid chromatography); Haemoglobin variants (HbS; HbE; thalassaemia)