Simultaneous Spectrophotometric Method for the Determination of Nimesulide and Tizanidine Hydrochloride

K. Dashora, S. Saraf and Swarnlata Saraf*

Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur - 492 010 (India)

The combination of nimesulide and tizanidine hydrochloride has been emerged as one of the widely prescribed combination in single dosage forms for anti-inflammatory and muscle relaxant. The nimesulide acts as a anti-inflammatory agent in combination with muscle relaxant tizanidine hydrochloride. The literature revealed that no method of simultaneous estimation by UV-spectrophotometer of both the drugs in tablet dosage forms have been reported till date. Hence a simple, rapid, accurate, economical and sensitive spectrophotometric simultaneous method, which is based on the additivity of absorbance for the determination of nimesulide and tizanidine hydrochloride in tablet formulation, has been developed. The Shimadzu Pharmaspec 1700 UV-Visible spectrophotometer with a matched pair of 10 mm quartz cells was used for the experimental purpose. The absorption maxima found to be at 390.5 nm and 319.5 nm respectively for nimesulide and tizanidine hydrochloride in methanol. Nimesulide obeyed the Beer Lambert’s law in the concentration range 5-35 μg/ml and tizanidine hydrochloride 5-30 μg/ml. The method was employed for the estimation of drugs in two marketed tablet formulations. The result showed the close proximity to the percentage of label claim. The method was validated with the recovery study and the result was reproducible. No interference was observed with excipients.

Nimesulfide; Tizanidie hydrochloride