Abstract

Praveen Kumar^1*, Chetna Tyagi¹, and Tejpal Singh Chundawat²

DOI : http://dx.doi.org/10.13005/ojc/410108

There are number of antibiotics or drugs which are found non-effective against various diseases caused by specific pathogens. The current challenges of multi-drug resistance (MDR), bacteria have compelled to find alternative antibacterial agents. Bacteriophages as lytic virus for bacteria seem a potential candidate to develop alternative antimicrobial agents. Bacteriophage specificity towards bacteria make it unique tool to counter bacterial infection and pathogens. In current comparative study, an attempt was made to isolate and screen bacteriophage against bacterial strains from different water sources as sewage, rain and pond against Pseudomonas strain (Gram negative) and Staphylococcus strain (Gram positive). Water samples were filtered by using of 0.45μM and 0.2μM syringe filters. Double layer agar method was used to isolate specific bacteriophage. Number of plaques/concentration of Bacteriophage is observed better with sewage water sample filtered through 0.45μM pore size syringe filters. For further characterization, isolated bacteriophage was enriched in liquid culture and further concentrated through using filter membrane of 0.45μM. Further studies will be carried out to standardize the optimal viral dose to control growth of its respective host rapidly and to check its cross activity.

Bacteriophage; Comparative study; Double layer agar method; Liquid method; Pseudomonas strain; Staphylococcus strain

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