Abstract

Kafui Kpegba¹, Kodjo Eloh², Kodjo Selom Evenamede^1*, Yaovi-Gameli Afanyibo³, Abdelhakim Elomri⁵, Oudjaniyobi Simalou¹, Pakoupati Boyode¹, Amegnona Agbonon and Elisabeth Seguin⁴

DOI : http://dx.doi.org/10.13005/ojc/350608

In Togo, the abusive use of the root of Cassia sieberiana D.C. in traditional medicine, contributes gradually to the rarefaction of the species. The general objective of this study is to promote the use of vital organs of Cassia sieberiana in traditional medicine in Togo. The identification of secondary metabolites of the extracts (cyclohexane, dichloromethane and methanol) was carried out by GC-MS and by CL-MS / MS. The antibiotic susceptibility test was performed according to the well diffusion method and the MICs and MBCs according to the tube dilution method. Compounds such as sitosterol α-acetate, β-sitosterol, emodin, chaetochromine, luteolin, (±) -catechin, naringenin 5-O-rhamnoside, guibourtinidol- (4 alpha-> 6) -catechin and (-) - epiafzelechin are found in the root and in the stem bark. The identified molecules give the different methanolic extracts, an antibacterial effect on all the germs tested. At the end of this study, it appears that the chemical composition of the stem bark is almost similar to that of the root bark. The leaves would be better placed for the treatment of bacteria tested.

Cassia Sieberiana; GC-MS; LC-SM/SM; Chemical Composition; Antibacterial Activity

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