Abstract

Nadezhda A. Byzova, Anatoly V. Zherdev and Boris B. Dzantiev*

DOI : http://dx.doi.org/10.13005/ojc/350603

This paper considers the combination of several immunochemical interactions for multiplex immunochromatographic analysis. It was shown that varying the composition of the gold nanoparticles–antibodies conjugate, its concentration and concentration of the hapten–protein conjugate changes both the maximal binding in the test zone and the range of the determined concentrations of the target analyte. Thus, it becomes possible to compensate for differences in the interaction times for the reagents of different specificities that arise when they are combined on one test strip. These regularities were demonstrated for multiplex analysis of three antibiotics. The developed assay has limits of detection of 10, 500, and 10 ng/mL for chloramphenicol, streptomycin, and ampicillin, respectively. Its working ranges for these three analytes are 0.5–6, 16–250, and 0.4–5 ng/mL. The analysis time is 15 min; the accuracy of the photometric determination of antibiotics in the working ranges is up to 6%.

Antibiotics; Colloidal Gold; Immunoassay; Lateral Flow; Test Strip

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