Abstract

Y. C. Wong* and Z. K. Lam

DOI : http://dx.doi.org/10.13005/ojc/350408

This study was investigating the production of bioethanol from the mixture of guinea grass (Panicum maximum) and using ragi tempeh through fermentation. The acid pretreatment process was carried out using 15% v/v sulphuric acid. The fermentation was carried out in anaerobic condition using bioreactor and shaker at 37°C and different pH of 5,6,7, and 8. Freezing test and Tollens’ test result showed very ethanol concentration in the sample product is very low, and aldehyde compounds are present in the product sample. The aldehyde is released due to degradation of lignocellulosic feedstock by acid hydrolysis. HPLC is carried out for getting a more accurate result. The best result is chromatogram of sample 5, obtained by fermentation using shaker with pH 8. It shows normal chromatogram with ethanol yield of 7.89%.

Acid Pretreatment; Bioethanol; Panicum Maximum; Ragi Tempeh

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