Abstract
Determination of Riociguat by Oxidative Coupling Using Visible Spectrophotometry
Giri Prasad Gorumutchu1, Venkata Nadh Ratnakaram*2and Sireesha Malladi3
DOI : http://dx.doi.org/10.13005/ojc/35Specialissue107
Abstract:
A simple spectrophotometric method was developed to determine riociguat in bulk and tablet formulation. The present method lies on the oxidation of MBTH by Fe+3 ions in acidic medium to form active coupling species and followed by its coupling with riociguat to form the chromophore having lmax 660 nm. Validated the proposed method as per the existing guidelines of ICH. Good linearity (r~ 0.999) was observed for calibration curve in the studied concentration range (6.25 – 37.50 μg mL-1). Reproducibility, accuracy and precision of the method were confirmed from low values of % RSD.
Keywords:MBTH; Oxidative Coupling; Riociguat; Validation; Visible Spectrophotometry
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