Abstract

Hasan Y. Muti¹ and Suleiman Olimat²

DOI : http://dx.doi.org/10.13005/ojc/340605

The main objective of this study was to establish a chromatographic method for analysis, determination and standardization of the two main components vanillic acid and luteolin as major components in Paronychia argentea Lam dry extract. This analytical method was designed to be a simple and fast with an appropriate separation of the two main components of the extract. High pressure liquid chromatography (HPLC) method of analysis was developed to quantitatively determine, identify and standardize the two main active constituents in the pharmaceutical dry extract against luteolin and vanillic acid as primary reference standards as it is the major active constituents of the dry extract of P. Argentea, where the linearity obtained was higher than R² = 0.99981 and 0.99908 respectively. Although the method was proven to be suitable, further specific analysis validation was conducted to include the following: linearity, precision, range, limit of detection, limit of quantitation and filter compatibility. The luteolin and vanillic acid were completely separated from the other components in the herbal dry extract with an R_f value of 1.3 and 5.7 minutes respectively. The concentration of Luteolin is 0.4% while vanillic acid content is 0.1% in the dry extract.

Analysis; Extract; Paronychia Argentea; HPLC; Linearity; Luteolin; Precision and Repeatability; Quantitation; Validation; Vanillic Acid

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