Abstract

Aminah Dalimunthe¹, Poppy Anjelisa Zaitun Hasibuan¹, Jansen Silalahi², Siti Fatimah Sinaga¹ and Denny Satria³

DOI : http://dx.doi.org/10.13005/ojc/340270

Litsea cubeba (Lour.) heartwood. was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane, chloroform at pH 3, 7, 9 and 11 to obtained alkaloid fractions. Isolation of alkaloid compounds were isolated with preparative thin layer chromatography. Antioxidant activity for fractions and isolates were determined with DPPH and ABTS assay. The highest IC₅₀ were given by chloroform fractions at pH 7 and compound III. They IC₅₀ were 23.81 ± 0.01; 3.12 ± 0.02 µg/mL for DPPH assay and 56.43 ± 0.06; 8.62 ± 0.02 µg/mL for ABTS assay. Compound III was showed maximum absorbance at (302.8; 280.8; 206 nm) and wave number (3286.70; 3109.25; 2939.52; 2835.36; 1589.34; 1462.04; 1365.60; 1246.02; 1018.41; 879.54 cm^-1) which indicate its an alkaloid compound with hydroxyl, methyl, methylene and methoxy group. The results reveal that alkaloid fractions and compounds of Litsea cubeba heartwood has very strong antioxidant potential.

Antioxidant. Litsea Cubeba; Heartwood; Alkaloid; Fractions; Compounds

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