Abstract

Sarithamol S, V. L. Pushpa and K. B. Manoj

DOI : http://dx.doi.org/10.13005/ojc/340255

Asthma is the most severe condition of lung airway inflammatory cells. Two third of asthmatic patients are nonresponsive towards existing treatments. The present study has focused on potency of IL4-JAK1-STAT6 signaling pathway as an investigational therapeutic target for the disease. Pomegranate plant is well studied for its potential phytochemicals. The therapeutic efficiency of pomegranate plant leaves and its active component ellagic acid against the signaling pathway has been investigated. The quantitative estimation of ellagic acid in the aqueous alcoholic extract of pomegranate leaves was done. Cell toxicity of  ellagic acid and plant leaf extract was checked on macrophage raw cells using MTT assay and their activity against JAK1 enzyme were found out by ELISA method. The result signifies that pomegranate leaf extract is more efficient than ellagic acid towards JAK1 enzyme. Therefore the present study has highlighted the potency of pomegranate leaf extract over ellagic acid, the active component, for the disease asthma through IL4-JAK1-STAT6 pathway.

Asthma; JAK1-STAT6 Pathway; Phytochemical Extraction; HPLC; MTT Assay; Activity Assay

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