Abstract

Wilaiwan Simchuer and Prasong Srihanam

DOI : http://dx.doi.org/10.13005/ojc/340235

Reports on phytosterol in wild grape (Ampelocissus martinii Planch.) fruits are rarely available worldwide. This work investigated the total triterpenoid content (TTC) and total sterol content (TSC) in skin and seed extracts from different growth stages of wild grape fruits using a spectrophotometric assay. In addition, we analyzed some free sterols using high performance liquid chromatography (HPLC). The mature fruit showed the highest content of TTC in both the skin and seed extracts. The TSC content was found the highest from skin extract in the ripe stage. The two most common phytosterols found were stigmasterol and β-sitosterol. The stigmasterol content was found the highest in the skin extract of the immature, while β-sitosterol was found in the seed extract of the ripe. This work indicated that the skin and seeds of wild grape fruits might be new sources of phytosterol.

Wild Grape; Phytochemical; Phytosterol; Spectrophotometric Assay

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