Abstract

Sultanat ^1,*, Ayaz Mahmood Dar¹, Asim Rizvi², Imrana Naseem²

DOI : http://dx.doi.org/10.13005/ojc/300322

Improved synthesis of 3b-acetoxy-9, 10-seco-19, 8(8)-spiro-5(10), 6-cholestadiene has been reported after reacting cholecalciferol acetate with dimethylbutadiene by incorporating BF₃·OEt₂, SnCl₄, ZnBr₂, p-TsOH in toluene under Diels-Alder condition to get better yields. Agarose gel electrophoresis showed the potential in vitro DNA damaging nature while as the comet assay depicted the genotoxic nature by mobilizing the tail of the comet in lymphocytes. The molecular docking depicted the intercalation of steroid derivative with minor groove of the DNA molecule and in this configuration the phosphodiester bond of DNA stabilizes the acetoxy group. The bioactivity score and PASS software analysis confirmed the potential physicochemical features of the compound to act as active drug.

cholestadiene; cholecalciferol; comet assay; gel electrophoresis; docking

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