Abstract

A.Vyas*, S. S. Shukla, R. Patel, R. Pandey, V. Jain, D. Singh and B. P. Nagori

A spectrophotometric method has been developed for the quantitative estimation of cephalexin in API and in tablets. The method is based on the analysis of complex, formed on the reaction of cephalexin with 1-floro-2, 4-di nitro benzene. The absorbance maxima (l-max) was found to be 441.2 nm. Factors affecting the reaction viz. reaction time, temperature and effect of reagent were optimized. The proposed method was validated as per ICH guidelines. The recovery studies confirmed the accuracy and precision of the method.

Cephalexin; 1-floro-2; 4-di nitro Benzene; Validation; Spectrophotometry

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