Abstract

A.Vyas, V. Jain, D. Singh, M. Singh, S. S. Shukla, R. Pandey, Saraf Swarnlata and S. Saraf

The present study was to develop the fingerprint method for Trikatu churna by simple high-performance thin layer chromatography (HPTLC) determination using piperine as a standard, which is as an important and major content in formulation. HPTLC methods for determination of piperine from the Trikatu churna along with its raw materials have been developed. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra - day assay precision, repeatability of measurement, and repeatability of sample application. The concentration of piperine present in raw materials was found to be 4.2%± 0.43w/w in Piper nigrum (Marica), and 2.15% ± 0.68w/w in Piper longum (Pipali) respectively and in three identical laboratory batch of Trikatu churna name TK-I, TK-II, TK-III, was 2.13%±0.62, 2.42%±0.67, 2.18%±0.41 w/w respectively with mean value 2.24%±0.48 w/w. The piperine content of all the three batches is found to be in close proximities with each other. Obtained results were compared with marketed formulations.

Piperine; Trikutu Churna; Ayurvedic Formulation; Fingerprint; Validation; HPTLC; Quality Control

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