Abstract

Shaker J. Azhari

A smpile, rapid and accurate method for the determination of main mono and divalent inorganic anions in Roselle (Hibiscus sabdariffa L.) has been developed. The quantitative determination of anions F-, Cl-, NO-2, Br-,NO-3, HPO2-4,SO2-4, and I- was accomplished by suppressed conductimetric ion chromatography (IC). The separation is achieved on shim-pack IC-SA2 250 mmL X 4.0 mm ID Peak. column operated at 30°C within 12.21 minutes by isocratic elution with 1.8 mM  potassium carbonate, 12 mM Potassium bicarbonate and 0.0125mM of N- {[(ethylamino) thioxomethyl] hydrazine carbonlmethyl} trimethyl ammonium chloride (ETHTC) and pH 9.25 as eluent at flow rate of 1ml/min. The method does not need a special sample treatment.

Optimization; determination; inorganic anions; ETHTC; Roselle (Hibiscus sabdariffa L) suppressed ion chromatography

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