Abstract

F. Shokraneh¹,  A. Dabirsiaghi^1* and N.  Adib²

A reliable, simple and sensitive reversed-phase high-performance liquid chromatographic method was developed for determination of losartan in plasma .Separation is achieved by HPLC after direct injection on a CN (250*4.6 mm) analytical column with a mobile phase composed of sodium hydrogen phosphate buffer - acetonitrile - tetrahydrofurane - methanol and phosphoric acid (0.1:5 :4 :21 :69.9 ) V/V% adjusted to pH= 9.9. Detection is by ultraviolent absorbance at 254nm. The flow rate was set at 0.6 ml/min. The lower limit of quantitation was 5 ng/ml. The intra and inter-day precisions (CV %) of the quality control samples were 0.57-5.31% and 0.21 -4.52% respectively. The recovery of method was 92.25± 2.19. The method was applied to a bioequivalence study in human.

Losartan; Human plasma; HPLC; Bioequivalence

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