Abstract

M. Padmalatha¹, K. Vanitha Prakash² And Eranna Dopadally³

Reversed phase high performance liquid chromatographic method was developed and validated for estimation of Atazanavir Sulfate in tablet dosage form. A Sunfire C18, 250x4.6 mm i.d, 5 μm partical size, with mobile phase consisting of a buffer of 0.05 M ammonium dihydrogen orthophosphate in water and acetonitrile in the ratio of 30:70 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 252 nm. The retention time was 4.78min. The detector response was linear in the concentration of 80-240mcg/ml, with the regression coefficient of 0.9999. Quantification was done by calculating area of the peak and the detection and quantitation limits were 0.04 and 0.12 mcg/ml respectively. The percentage assay of Atazanavir Sulfate was 98.73%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method can be applied for the determination of Atazanavir Sulfate in quality control samples and formulations without interferences of the excipients present.

Atazanavir sulfate; RP-HPLC; estimation and capsules

[ View HTML Full Text]