Abstract

Ajay Pratap Singh* and K. D. Sexena

Momardica charantia L.var. Jhalri (MCJ) is the member of lectins from the family Cucurbitaceae. For further characterization MCJ lectin was analyzed for amino acid identification and sugar analysis by paper chromatography in the present paper. The technique reported the presence of total 18 amino acids with large content of Serine and Glutamic acid. The same technique revealed the presence of glucose, mannose and hexosamine. The total hexose sugar by Orcinol- sulphuric acid method and total hexosamine by Elson and Moragn method was estimated to be 6.81mg and 5.037mg respectively per 100mg of MCJ lectin. The good content of carbohydrate in MCJ lectin being glycoprotein in nature was considered as a major and crucial factor for apparent high mol. Wt. of MCJ lectin.

Lectin; Paper chromatography; Sugar; Glycoprotein

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