Abstract

G. F. Patel*, N. R. Vekariya and H. S. Bhatt

A rapid and simple high performance thin layer chromatography (HPTLC) method with densitometry at 263 nm was developed and validated for simultaneous determination of lopinavir and ritonavir from capsule dosage forms. Separation was performed on aluminum-backed silica gel 60F254 HPTLC plates as stationary phase and using a mobile phase comprising of toluene, ethyl acetate, methanol and ammonia, in the volume ratio of 6.5:2.5:0.5:0.5 (v/v) respectively. After development, plates were observed under UV light. The detector response was linear in the range of 6.5 to 20.00 μg/spot and 1.5 to 5.00 μg/spot for lopinavir and ritonavir respectively. The limit of detection was found to be 1.5 ng/spot and 4.6 ng/spot and limit of quantification was found to be 21.00 ng/spot and 5.10 ng/spot for lopinavir and ritonavir respectively. The percentage assay of lopinavir and ritonavir was found between 99.90±1.45 and 101.29±1.95 respectively. The developed method was validated as per ICH guidelines and by recovery studies. The method was found to be simple, rapid, precise and accurate and can be used for routine analysis.

HPTLC; Lopinavir; Ritonavir; Simultaneous determination

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