Abstract

Rosnani Nasution^1*, Mustanir¹, Marianne² and Iswani Marzuki¹

DOI : http://dx.doi.org/10.13005/ojc/320542

The extract n-hexane and ethyl acetate obtained from plants Ficusracemosa, respectively of 68.50 g (2.28%) and 50.52 g (1.68%), were tested their antiobesity, with   dose of 100, 500, 1000 and 1500 mg / kg bw of mice. The results showed that  the extract n-hexane and ethyl acetate can weight loss in mice, obtained optimal dose is 1500 mg / kg bwof mice,   and used as   dosis of next work. Furthermore, 30 g of ethyl acetate extract were fractionated, and yielding three groups of fractions, namely fraction groups A, B and C.Testantiobesity against the fractions A, B, and C were carried out for 5 days each using three mice, can  decrease mice  weight of average : 2.89%; 2.57%; 0.56%, while 4.14% for  positive control, whereas the negative control mice  increase  weight  of mice as much as 4.82%. Fraction A, after separated with chromatography produce a compound suspected of β-amyrin acetate. Compounds        β-amyrin acetate at a dose of 1500 mg / kg bw of mice,  showed a weight loss of 2.31%. β-amyrin acetate compound was  characterized by Mass Spectrometry (MS), Proton Nuclear Magnetic Resonance (¹H-NMR) and Fourier Transform Infrared Spectroscopy (FT-IR).

anti-obesity; Ficusracemosa; isolation; β-amyrin acetate

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