Abstract

Giridhar Goudar¹ and Sathisha G. J¹*

DOI : http://dx.doi.org/10.13005/ojc/320458

Foxtail millet (Setaria italica) grain cultivars were analyzed for the phenolic components which have beneficial effects on human health. The aim of the present study was to evaluate the effect of dehusking on total phenolic and total flavonoid contents in four types of foxtail millet grain varieties. These grains were extracted for phenolic compounds and evaluated for total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity. TPC was analyzed by Folin-Ciocalteu method, TFC by aluminium chloride method and antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. High Performance Thin Layer Chromatography (HPTLC) evaluation was done for ferulic acid in the phenolic extracts, identification was done by comparing R_f value of the ferulic acid standard with the sample extracts and quantification was done using the peak areas of the obtained chromatograms. Results revealed that TPC, TFC and antioxidant activity was found to be high in husk of the grains compared to whole grains and dehusked grains, whereas when compared amoung different cultivars Black variety got the high content of phenolics.

Foxtail millet; phenolic; flavonoid; ferulic acid; HPTLC

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