Abstract

Narjis Naz¹, Aiza Kashif¹, Warda Sheikh¹,Mateen Abbas² and Abdul Muqeet Khan²

DOI : http://dx.doi.org/10.13005/ojc/320221

The present study provides information about the levels of fortification of three water soluble vitamins i.e. thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in a variety of foodstuffs include milk products and cereals for young children. Food fortification is key implement for improving health of the growing children. Twenty food samples were chosen for analysis because of their common utilization in the local area. The vitamin concentrations were determined by high performance liquid chromatography  with C₁₈ column with a gradient of mobile phase made of water and acetonitrile and a diode array detector set at 280 nm. The thiamine content investigated in the samples ranging from 268 µg/mL to 3 µg/ml, nicotinamide content was from 41 µg/ml to 1 µg/mL while the pyridoxine level was in between 412 µg/mL  to 20 µg/mL. Detection and Quantification of compounds were attained by comparing their retention times with  standard reference materials and on the basis the off peak area match against those of a standard. The method used, offer excellent linearity with r² ≥ 0.994, detection limits, reproducibility, and analyte recovery.

Water soluble vitamins, Thiamine, Nicotinamide, Pyridoxine, High performance liquid chromatography, Food products

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