Abstract

Alka Srivastava²*, Rashmi Mohan Mathur¹, Rachna Prakash¹ and Shashi Agrawal¹

DOI : http://dx.doi.org/10.13005/ojc/320250

Healthy and bold seeds of castor variety “Aruna“ were collected. The de-hulled castor kernels were separated from seed coat and finely grounded in an electric grinder. The powdered kernels were passed through 60 mesh sieve and defatted using n-hexane in a soxhlet extractor. After complete extraction of oil seed meal was made free from solvent at low temperature. The defatted seed meal was pulverized and again passed through 60-mesh sieve to get final product .The RICINUS LIPASE source thus, obtained was stored in a screw capped glass vial and kept in a desiccator. As and when required portions from this preparation were used in further enzymatic studies.

Ricinus Lipase; Germinated castor seeds; Cotyledons

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