Abstract

Naser Montazeri*, Khalil Pourshamsian, Zahra Barami and Safar-Ali DOrrieh

The aim of this study is to provide a simple, rapid access and commodious technique used in the quantitative analysis of analytes and requires less sample handling and also lowering the cost of analysis by reducing both the amount of solvent necessary to prepare a sample and the amount of waste requiring disposal. In this headspace solvent microextraction coupled with hydrodistillation method a droplet of n-Hexadecane containing n-Octadecane as an internal standard was used for extraction. Analytes were extracted by suspending a 2 µL droplet directly from a microsyringe tip over the surface of the solution. After extraction, the droplet was retracted back into the syringe and injected directly into a GC injection port. The effect of different parameters on extraction efficiency were optimized using a one at a time optimization method, including: extraction solvent (n-hexadecane), sample mass (0.1 gr), microdrop volume (2 µL) and extraction time (6 min). On the analyte peak-to-internal standard peak ratio have been studied. The volatile components were studied both by HD-HSME and an ordinary hydrodistillation method and the main components identified were, α-pinene, 1, 8-cineol, camphene and myrcene in both methods. The results were in good correlation in comparison with the hydrodistillation method. By HD-HSME twenty compounds were identified. á-Pinene (46.5%), 1, 8- cineole (13.4%), camphene (12.9%) and myrcene (4.1%) were found to be the major constituents.

Headspace solvent microextraction coupled with hydro-distillation method (HD-HSME); microsyringe; microdrop; hydrodistillation (HD); internal standard

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