Abstract

Masoomeh Afzali Poor Khoshkbejari¹, Alireza Jafari*², Mojhdeh Safari³

DOI : http://dx.doi.org/10.13005/ojc/310322

Urinary tract infection has change a basic challenge in human population. Antibacterial nanoparticles have turned over the new leaf in scientist's research.Ag/ZnO nanoparticleis synthesis in high temperature (500⁰C).FT-IR, XRD, SEM and TEM were used for determination of spectroscopic, structural and morphology of samples, respectively. Also the nanoparticle was digested and analyzed by ICP-AES for determining the presence of residual chemical element in the nanoparticle. Bacterial sensitivity to nanoparticle was commonly tested using by MIC and MBC tests. For achievement of in vivo tests, the renal of rats were injected and then were infected with E.coli. After that, animals were divided to 9 treatment group and the effect of three concentrations of MIC and MBC; also intermediate concentrations of MIC and MBC and each as a separate group, were studied.The particles size was less of 12nm, approximately. The MIC and MBC observed for Ag/ZnO were 32µgml^-1and 512µgml^-1 for E.coli, respectively.Treatment with a concentration 512µgml^-1ofAg/ZnO nanoparticle in combination with Ciprofloxacin reduced severity of renal cortical thickness of pyelonephrit is rats.The microscopic pathological examination indicated that there was no any severe Inflammation of renal tubules in the groups that had been treated with 512µgml^-1concentration of Ag/ZnO nanoparticlein combination with ciprofloxacin. In compared with the control groups.This study showed thatthe Ag/ZnO nanoparticle in cooperation with ciprofloxacin has great antimicrobial effect against E.coli on renal damage of Pyelonephritis.

Ag/ZnO Nanoparticle; Antibacterial; Pyelonephritis

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