ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Abstract

Phytochemical, Antimicrobial and Total Phenol Test of Coral Plants “Betadin” Leaf Methanol Extract (Jatropha Muitifida Linn)

Hanafi, Septilina Melati Sirait*, Sri Redjeki Setyawatiand Lilis Sulistiawaty

DOI : http://dx.doi.org/10.13005/ojc/360330


Abstract:

Coral Plants leaf was extracted with methanol and carried out by maceration then evaporated with a rotary evaporator. This extract contained several phytochemical compound such as alkaloid, flavonoid, tannin, phenol and terpenoid. Therefore, Coral Plants leaf has an antimicrobial function. Phenol compound has the ability to form complex compound with proteins through hydrogen bonds, which can damage bacterial cell membrane. Total phenol content was quite high in the leaf of 165.20 ± 33 mg GAE / mL. Antibacterial activity test results showed that the methanol extract of Coral Plants leaves can inhibit E. coli gram-negative bacteria with strong inhibition (10-20 mm) and gram-positive bacteria (Bacillus sp) by 12.3 mm. Coral Plants leaves can inhibit the growth of penicillium and Tricodherma fungi, but cannot inhibit the growth of Aspergillus niger fungi. The inhibition against Penicillium was higher (7 mm) compared to Tricodherma of 1.8 - 4.5 mm

Keywords:

Antimicrobial; Coral Plants; Leaf; Phytochemical; Total Phenol

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