Abstract

Muazzam Sheriff Maqbul¹, Ali Mohamed Alshabi², Aejaz Abdullatif Khan³, S.M. Shakeel Iqubal^3*, Ibrahim Ahmed Shaikh⁴, Tasneem Mohammed³, Mohammed Shafiuddin Habeeb⁴, Yumna Abdulmalek Bokhari⁵, Kayamkani Abedulla Khan⁶ and Mohammed Shahid Hussain⁷

DOI : http://dx.doi.org/10.13005/ojc/360318

The World Health Organisation (WHO) has stated that about fifty percent of the world population is inflicted with the gastro-duodenal diseases due to contamination food and water where antibiotics are the only choice of treatment. The abundant use of antibiotics also has resulted in the emergence of mutant multi drug resistant organisms. The starting point of a revolution concerning the concepts for the management of gastro-duodenal diseases was initiated upon the discovery of Gram negative helical shaped bacterium known as Helicobacter pylori (H. pylori) in the year 1982. In this study Polymerase chain reaction (PCR) technique was used to detect its 16S rRNA gene sequence analysis unambiguously. This study is a small effort to resurrect the ancient phyto-pharmaceutical substances to treat such infections by comparing the antimicrobial efficacy alongside bioactive composition analysis of the dried seed oils of Moringa oleifera (M. oleifera) from Indian origin and Moringa peregrina (M. peregrina) from Egyptian origin. The phenolic content in these seed oils almost serves as the potential antimicrobial effect towards combating the pathogens and preventing the infection to curb the diseases. The biochemical components present in the dried seed oils of M. oleifera and M. peregrina were determined by GC-MS analysis. The antimicrobial efficacy of the seed oils were studied using the standard antibiotic assay technique which determined that the sensitivity of the bacterium towards both the seed oil was excellent when compared to that of the eight standard chemical antibiotics used for the comparative study. The minimal concentration value of these seeds shown promising results to inhibit or completely eradicate the bacterium when compared to the values of the eight standard chemical antibiotics used for the comparative study.

Antimicrobial Activity; GC-MS; Helicobacter Pylori, Moringa Seed Oil; Moringa Oleifera, Moringa Peregrina, PCR

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