Abstract

Supawadee Patathananone*¹, Jureerut Daduang², Amonrat Koraneekij² and Chia-Ying Li³

DOI : http://dx.doi.org/10.13005/ojc/350302

The usage of ripe hog plum fruit (Spondias pinnata) extracts in cosmetics and food products, including cancer therapeutic agents, have a few studies. Herein, the strong anti-tyrosinase activity found in the extracted part of isopropanol is reported. This extract was separated by liquid/liquid extraction using hexane: methanol+H₂O. The hydrophilic layer (6A*) exhibited the anti-tyrosinase, antioxidant, and anticancer activities in vitro. The IC₅₀ value of each bioactivity was presented as approximately 0.18, 0.04, and 1.40 mg/ml, respectively. In addition, 6A* fraction showed a very low cytotoxic effect in normal fibroblast cells (NHDF cells). The bioactive agents in 6A* were purified by C18 reverse-phase High-Performance Liquid Column Chromatography (HPLC). The 12 purified peaks were shown in the chromatogram profile. All peaks (excepted 6A-06 and 6A-09) displayed anti-tyrosinase activity, whereas the antioxidant property was not found in 6A-01, 6A-06, and 6A-08 but was represented in other peaks. Most purified peaks were indicated to be the aromatic alcohol or derivative phenol compounds.

Cytotoxic Effect; Flavonoid; Phenolic Acid; Tyrosinase Inhibitor; Wild Mango

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