Abstract

Ashwani Kumar and Mamta Kamboj

DOI : http://dx.doi.org/10.13005/ojc/340634

A rapid and sensitive spectrophotometric method for the determination of vitamin A and vitamin E using Fe(II)-1-nitroso-2-napthol detection system is reported. The in situ reduced Fe(II) formed green coloured complex with 1-nitroso-2-napthol which gives an absorption maxima at 708nm. Beer’s law was obeyed in the range 4.0-14.0μg/mL for vitamin A and 4.0-24.0μg/mL for vitamin E with relative standard deviation 1.7% for vitamin A and 2.2% for vitamin E. The molar absorptivity was found to be 1.3×10⁴ L mol^-1 cm^-1 for vitamin A and 2.5×10⁴ L mol^-1 cm^-1 for vitamin E with the co-relation coefficients 0.996 and 0.935 for vitamin A and vitamin E respectively. Optimization of various factors was done to get the maximum absorption value. The proposed method is utilized satisfactorily for pharmaceuticals, milk sample,fruit sample and different liquid samples of vitamin A and vitamin E by pre treatment of samples.

Vitamin A; Vitamin E; Spectrophotometeric Quantitation

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