Abstract
Comparison of Iron Reduction Methods on the Determination of Antioxidants Content in Vegetables Sample
Yefrida, Hamzar Suyani, Hermansyah Aziz and Mai Efdi
DOI : http://dx.doi.org/10.13005/ojc/340525
Abstract:
Determination of antioxidant content in 14 vegetables has been done using Modified Phenanthroline (MPM), Phenanthroline (PM) and Ferric Reducing Antioxidant Power (FRAP) methods. The SDR values obtained for the MPM and PM methods were (2.65 ± 1.26) % and (3.51 ± 0.88) %, while the recovery values were (100.86 ± 4.45)% and (99.93 ± 3.50) %, respectively. The antioxidant content of vegetables obtained by MPM, PM and FRAP methods were (30.4 ± 1.46 - 515.9 ± 3.32), (25.9 ± 1.13 - 327.3 ± 2.57) and (21.4 ± 0.51 - 371.7 ± 1.10) μmol Fe / g DW, respectively. The antioxidant content obtained with the three methods showed no significant different. The correlation values between MPM - PM, MPM - FRAP, and PM - FRAP methods are 0.9712, 0.9896 and 0.9611, respectively. These values indicate a very strong correlation among the three methods.
Keywords:Antioxidant Content; FRAP Method; Iron Reduction Method; Modified Phenanthroline Method; Phenanthroline Method; Vegetables
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