Abstract

Ace Baehaki^1*, Shanti Dwita Lestari¹, Nuni Gofar² and Yudi Wahidman¹

DOI : http://dx.doi.org/10.13005/ojc/320647

The  purpose of this study wasto partially characterize chitosanase isolated from snakehead fish’s (Channa striata) digestive tract.Two types of snakehead fish, domesticated and non-domesticated, were used as samples in order to assess the effect of feeding types on chitosanase activity. Chitosanase isolated from the stomach andintestine of domesticated snakehead fish had higher activity than non-domesticated samples with the value of 0.0395 U/mL and 0.0296 U/mL, respectively. The optimum chitosanase activity from the stomach and the intestine of domesticated fish was at pH 7. Lower optimum pH (6) was noticed for non domesticated samples. Chitosanase from both samples showed optimum activity at 70 °C.

characterization; chitosanase; digestive tract; snakehead

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