Abstract

Thuttagunta Manikya Sastry¹*, Karripeddi Ramakrishna² Kotturu Venkata Lavanya³ and Kallempudi Siva Iva Prasad⁴

DOI : http://dx.doi.org/10.13005/ojc/300144

A sensitive visible spectrophotometric method was developed for quantitative determination of Antiviral [Abacavir sulphate (AVS)] agent against HIV in bulk material and dosage forms. An extraction spectrophotometric method for the assay of abacavir sulphate is based on the formation of colored co-ordination complex with cobalt thiocyanate (CTC) which can be extractable in nitrobenzene (l_max=620 nm) is described. Beer's law limits and Molar absorptivity values were found to be 10 – 50 mg mL^-1 and 9.58 x 10³ L mol^-1cm^-1 respectively. The limit of detection and limit of quantification values are found to be 3.07 x 10^-1 µg mL^-1 and 9.3 x 10^-1 µg mL^-1 respectively. Precision and accuracy of the developed method was evaluated. The results are validated statistically and compared with reported methods.

Spectrophotometry; Abacavir sulphate; Ziagen Nitrobenzene; Cobalt thiocyanate (CTC) and dosage forms

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