Abstract

K. V. Siva Prasada Rao, C. Srinivasulu, P. Nagaraju, S. R. Sagineedu and G. Prabhakar

Three visible spectrophotometric methods have been described for the assay of gatifloxacin (GAT) either in bulk form or dosage forms. Methods A-C are based on the oxidation of GAT with an excess of oxidant [N-bromosuccinimide (NBS) in methods A and B or chloramine-T (CAT) in method C] in acidic medium. The unreacted oxidant is then estimated colorimetrically by using an oxidisable dye [Celistine blue (CB) in method A or Gallocyanine (GC) in method C] or by p-N-methyl aminophenol sulphate (PMAP)- sulphanilamide (SA) reagent in method B. Regression analysis of Beer's law plots showed good concentration range 2.0-10.0 mg/ml, 4.0-20.0 mg/ml and 1.0-5.0 mg/ml for methods A, B and C respectively and gives reproducible results.

Spectrophotometry; NBS; CB; PMAP; SA; CAT; GC

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