Abstract

Homa Jilani Khan^1,2, Mohammad Kaleem Ahmad², Abdul Rahman Khan¹, Namrata Rastogi², Jamal Akhtar Ansari^1,2, Nishat Fatima^1,2,, G. N. V. Satyanarayan³, and Abbas Ali Mahdi^2*

DOI : http://dx.doi.org/10.13005/ojc/320213

The aim of this study is to characterize the chemical composition, in vitro antioxidant and anticancer activity of chloroform fraction of Swertia chirata L. (Family Gentianaceae). GC-MS analysis of chloroform fraction of S. chirata (CFSc) identified twenty-five compounds belonging to different chemical class. Among these important classes of compounds were tri-terpenoids, phenol, acids, and fatty acids. Our phytochemical analysis of CFSc was well supported by its Total Phenolic Content and antioxidant activity with in vitro anticancer activity which was evaluated by MTT assay also showed that CFSc exhibited anti-proliferative activity against human breast cancer cell lines (MDA-MB-231 and MCF-7). In conclusion; our study revealed an extensive biochemical profile of CFSc as well its potential as a pharmacological agent with respect of its antioxidant and anti-cancer property. More safety and toxicological studies are needed to be addressed to ensure the use of CFSc for medicinal purposes.

GC-MS; Tri-terpenoids; Phenols; MTT assay; Anti-proliferative

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