Abstract

Kanda Saosoong, Isarawat Litthanapongsatorn and Chalerm Ruangviriyachai^*

DOI : http://dx.doi.org/10.13005/ojc/320237

The correlation between antioxidant activity and total phenolic content of the crude extract from J. curcas fruit were evaluated. The crude extract was obtained by extracting with selected solvent and fractionated with a hydrophilic lipophilic balanced (HLB) cartridge. The total phenolic content of crude extract with Folin-Ciocalteu method gave the content at 7.04 ± 0.10 mg GAE/g of extract and 0.22-18.61 mg GAE/g of extract for its fraction. The antioxidant activity of crude extract was 270.98 ± 0.59 µmol Fe/g of extract with Phen method and the extract gave IC₅₀ at 14.09 ± 0.05 mg/mL with DPPH method. A good correlation among antioxidant activity and total phenolic content of both methods were observed. Furthermore, the methanolic fraction (F₅) showed highest antioxidant activity with IC₅₀ at 0.04 ± 0.02 mg/mL as the study of DPPH method and 207.53 ± 2.58 µmol Fe/g of extract as the study of Phen method.

Jatropha curcas fruit; Total phenolic content; Antioxidant activity; DPPH method; Phen method; Pearson correlation

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