Abstract

Suman Sharma¹ , Ashutosh Pareek¹ *, Richa Joshi¹ , Yashumati R. Bhardwaj¹ , Vivek Jain¹ and Gunjan Jadon²

The method has been developed and validated for the assay of Paracetamol using mixed solution of methanol and phosphate buffer 6.8 as a solvent in ratio of (1:3) (further diluted in phosphate buffer 6.8 only). The λ max (absorption maxima) of the drug was found to be 246 nm. Linear response was observed in the range of 2-24ìg/ml with a regression coefficient of 0.999. Furthermore various validation parameters as per ICH Q2B guideline were tested and found accordingly. The method and solution was significantly (P<0.05) stable for 15 days. The percent purity (99.09 %) and % Recovery at 80, 100 and 120 % were found to be 102.00±2.027, 102.10±4.913 and 100.00±4.819 respectively for marketed paracetamol tablets. Developed method was a less toxic, cheap, eco-friendly but equally sensitive spectroscopic method for quantitative determination of paracetamol for regular quality control purpose in laboratories.

Paracetamol; phosphate buffer 6.8; sandell's sensitivity; molar absorptivity

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