Abstract

Sayanna¹, T. Veeraiah^1* and Ch. Venkata Ramana Reddy²

DOI : http://dx.doi.org/10.13005/ojc/310360

Siimple and sensitive extractive spectrophotometric methods have been described for the determination  of Cyproheptadine hydrochloride (CPH) in pure form and in pharmaceutical formulations. The developed methods involved formation of coloured chloroform extractable ion-pair complexes of the CPH with triphenyl methane dyes viz.,  bromothymol blue (BTB), bromocresol green (BCG) and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima at 419, 419 and 405nm with use of the above dyes, respectively.  Beer’s law is obeyed in the concentration ranges 3.0-25, 2.5-22.5 and 1.5-25μg/ml with BTB, BCG and BCP respectively. The stoichiometry of the complex is found to be 1:1 in each case. The effect of concentration of dye, pH and interference of excipients have been studied and optimized. The limit of detection and limit of quantification have been determined for three methods. The methods are applied to the determination of CPH in commercial tablets and results of analysis are validated statistically through recovery studies also the methods are validated as per the guidelines of ICH.

Spectrophotometry; Cyproheptadine hydrochloride; Bromothymol blue; Bromocresol green; Bromocresol purple; Ion-pair complex; Validation

[ View HTML Full Text]