Validated Spectrophotometric Methods for the Determination of Tadalafil in Pharmaceutical Formulations

V. Naga Lakshmi¹, D.Ravi Kumar¹, S.V.M.Vardhan² and C.Rambabu²

¹Department of Chemistry, Acharya Nagarjuna University Nuzvid Campus, Nuzvid (India).

²Department of Biochemistry, Acharya Nagarjuna University Nuzvid Campus, Nuzvid (India).

 

ABSTRACT:

Two simple and sensitive UV-Visible spectrophotometric methods A and B for the determination of tadalafil have been described. These methods are based on the formation of bluish green color and blue colored chromogens obtained when the drug was condensed with Isatin and Xanthydrol exhibiting lmax at 665 and 640nm respectively. These methods obey Beer’s Law in the concentration ranges of 0.4-2.0 and 4-20 μg/mL. The optimum conditions for all color development are described and the proposed methods have been successfully applied for the determination of tadalafil in bulk drug and pharmaceutical formulations. The common execipients and additives did not interfere in this method.

KEYWORDS:

Tadalafil; chromogen; spectrophotometry; pharmaceutical

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Copy the following to cite this article: Lakshmi V. N, Kumar D. R, Vardhan S. V. M, Rambabu C. Validated Spectrophotometric Methods for the Determination of Tadalafil in Pharmaceutical Formulations. Orient J Chem 2009;25(3).

Copy the following to cite this URL: Lakshmi V. N, Kumar D. R, Vardhan S. V. M, Rambabu C. Validated Spectrophotometric Methods for the Determination of Tadalafil in Pharmaceutical Formulations. Orient J Chem 2009;25(3). Available from: http://www.orientjchem.org/?p=23173