Extractive Colorimetric Method for The Determination of Saquinavir Mesylate in Dosage Form

M. Padmalatha¹ and K. vanitha Prakash²

¹Vijaya College of Pharmacy, Munaganoor (V), Hyderabad - 501 505 (India).

²Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road No. 3, Banjara Hills, Hyderabad - 500 034 (India).

ABSTRACT:

Two simple, economical, precise, reliable and reproducible visible spectrophotometric methods (A and B) have been developed for the estimation of Saquinavir mesylate in bulk as well as in tablet formulation. The developed methods A and B are based on the formation of ion pair complexes of the drug with Bromocresol green (BCG) and Orange-II extractable into chloroform, which shows absorbance maxima at 435 nm and 486 nm respectively. The absorbance-concentration plot is linear over the range of 2.0-25 mcg/mL for method A and 5- 25 mg/mL for method B. The molar absorptivity, Sandell’s sensitivity, Ringbom optimum concentration ranges for the above complexes were calculated and evaluated at their maximum wavelengths. The different experimental parameters affecting the development and stability were studied carefully and optimized. Results of analysis for all the methods were validated statistically and by recovery studies.

KEYWORDS:

Saquinavir mesylate; bromocresol green; Orange-II; Ultraviolet-Visible double beam spectrophotometer

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Copy the following to cite this article: Padmalatha M, Prakash K. V. Extractive Colorimetric Method for The Determination of Saquinavir Mesylate in Dosage Form. Orient J Chem 2009;25(2).

Copy the following to cite this URL: Padmalatha M, Prakash K. V. Extractive Colorimetric Method for The Determination of Saquinavir Mesylate in Dosage Form. Orient J Chem 2009;25(2). Available from: http://www.orientjchem.org/?p=23231