Simple and Rapid Methods for the Analysis of Sulfonamide Bacteriostatic Antibiotic in Dosage Forms

G. Vijaya Raja¹*, C. Bala Sekaran², P. Siva kumari² and S. K. Parvee²

¹Department of Biotechnology, Acharya Nagarjuna University, Guntur (India).

²Department of Biotechnology, P.B. Siddhartha College of Arts & Science, Vijayawada - 520 010 (India).

ABSTRACT:

Two novel simple, precise and rapid spectrophotometric methods (Mehod A and Mehod B) are proposed for the determination of sulfamethaxazole (SFZ) using ferric chloride and potassium ferricyaide, sodium nitrite and resorcinol, as reagents. Method A is based reduction of Fe+3 by SFZ and Fe+2 produced forms complex with potassium ferricyanide to form a green colored chromogen having maximum absorption at 720nm. Method B is based on the diazotization of the drug by sodium nitrite in acidic medium at 5°C followed by coupling with resorcinol to form yellow colored species (lmax 430nm). Beer’s law is obeyed in the ranges, 4-20 and 2-10 μg/mL for method A and method B, respectively. The apparent molar absorptivities are calculated to be 0.61 x 104 and 1.089

KEYWORDS:

Sulfamethaxazole (SFZ), Sandell’s sensitivity; Visible Spectrophotometric determination; Beer’s Law

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Copy the following to cite this article: Raja G. V, Sekaran C. B, kumari P. S, Parvee S. K. Simple and Rapid Methods for the Analysis of Sulfonamide Bacteriostatic Antibiotic in Dosage Forms. Orient J Chem 2008;24(3).

Copy the following to cite this URL: Raja G. V, Sekaran C. B, kumari P. S, Parvee S. K. Simple and Rapid Methods for the Analysis of Sulfonamide Bacteriostatic Antibiotic in Dosage Forms. Orient J Chem 2008;24(3). Available from: http://www.orientjchem.org/?p=25055