ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Purification of Hsc70 from Liver Tissue

Volume 24, Number 3

Aara Rifat, S. A. Hajam and K. I. Andrabi

Department of Biochemistry, University of Kashmir, Kashmir - 190 006 (India).

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ABSTRACT:

A 70KDa protein has been purified by anion exchange chromatography on (DE-52).Molecular weight of protein was also determined by employing 10% SDS-page with phosphorylase b(Mr 92,500) BSA (Mr 68000) ovalbumin(Mr 43,000) chymotrysinogen(Mr 27,000) and Myoglobin(Mr17200) as the marker proteins.After electrophoresis gel was stained with 0.25% CBB for 4 hour and were then destained.

KEYWORDS:

SDS DEAE; CBB

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Rifat A, Hajam S. A, Andrabi K. I. Purification of Hsc70 from Liver Tissue. Orient J Chem 2008;24(2).

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Rifat A, Hajam S. A, Andrabi K. I. Purification of Hsc70 from Liver Tissue. Orient J Chem 2008;24(2). Available from: http://www.orientjchem.org/?p=24956

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