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Purification and Identification of 35kda Heat Stable Protein

Volume 24, Number 3

Aara Rifat, S. A. Hajam and K. I. Andrabi

Department of Biochemistry, University of Kashmir - 190 006 (India).  

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ABSTRACT:

A heat stable protein (hsp) has been purified to homogeneity from sheep liver extract by heating to 95c followed by anion-exchange chromatography on DEAE-cellulose (DE-52). The purified protein showed a single band when examined by gel electrophoresis. The molecular weight determined by SDSpage was found to be approximately 35KDa. The presence of this protein was also demonstrated in whole cell homogenate, heat stable and nuclear fraction.

KEYWORDS:

Hsp; DEAE; SDSpage

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Rifat A. Hajam S. A, Andrabi K. I. Purification and Identification of 35kda Heat Stable Protein. Orient J Chem 2008;24(2).

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Rifat A. Hajam S. A, Andrabi K. I. Purification and Identification of 35kda Heat Stable Protein. Orient J Chem 2008;24(2). Available from: http://www.orientjchem.org/?p=24978

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