ISSN : 0970 - 020X, ONLINE ISSN : 2231-5039
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Inhibition of Glycine Max Urease by Chlorophenols

Volume 21, Number 3

Dilip B. Patil* and Anand A. Kshirsagar

Department of chemistry, Institute of science, Nagpur - 440 001 (India)

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ABSTRACT:

The inhibition of the glycinemax urease catalyzed hydrolysis of urea by phenol, o-, p-Chlorophenol and 2, 4-Dichlorophenol has been carried out on the basis of Kinetic measurement of urea hydrolysis. The concentration of urea was varied in the range of 0.40 x 10-3M. The apparent Michaelis constants, Kmapp and dissociation constant of Enzyme – inhibitor complex, Ki were determined at pH 6.80 and temprature 37.0°C. All the chlorophenols shows competitive type of inhibition.

KEYWORDS:

Glycine max; Urease; Chlorophenols; Phenolic Inhibitors

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Patil D. B, Kshirsagar A. A. Inhibition of Glycine Max Urease by Chlorophenols. Orient J Chem 2005;21(3).

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Patil D. B, Kshirsagar A. A. Inhibition of Glycine Max Urease by Chlorophenols. Orient J Chem 2005;21(3). Available from: http://www.orientjchem.org/?p=19299

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