Study of Inhibiting Action of Some Nitrobenzoic Acids on Urease Catalysed Hydrolysis of Urea

Dilip B. Patil

Department of Chemistry, Government Science College, Gadchiroli - 442 605 India.

ABSTRACT:

The inhibiting action of benzoic acid, o-nitrobenzoic acid, m-nitrobenzoic acid, p-nitrobenzoic acid and 3,5-dinitrobenzoic on urease catalysed hydrolysis of urea has been investigated on the basis of kinetic measurement. The concentration of urea was varied in the range of 1.31 x 10-3 M............3.33 x 10-3 M and that of inhibitor was maintained 2.71 x 10-3 M The Michaeiis constant and dissociation constant of enzyme-inhibitor complex were determined. The pH and temperature of the reaction system were 6.70,37°C respectively. Benzoic acid and all nitrobenzoic adds have shown competitive type of inhibition.

KEYWORDS:

m-nitrobenzoic; p-nitrobenzoic; dinitrobenzoic urease

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Copy the following to cite this article: Patil D. B. Study of Inhibiting Action of Some Nitrobenzoic Acids on Urease Catalysed Hydrolysis of Urea. Orient J Chem 2000;16(3).

Copy the following to cite this URL: Patil D. B. Study of Inhibiting Action of Some Nitrobenzoic Acids on Urease Catalysed Hydrolysis of Urea. Orient J Chem 2000;16(3). Available from: http://www.orientjchem.org/?p=36197