Abstract

Panikumar Durga Anumolu¹, Syed Sara Afreen^1*, Gokara Madhuri¹,  Vijaya Durga Dumpala¹, Swathi Naraparaju ²andVeera Shakar Pulusu²

An unambiguous, precise, and accurate approach was created for the contemporaneous assessment of Meropenem (MPM) and Vaborbactam (VBM) in pharmaceutical dosage form and bulk. For the chromatogram, Standard  Zorbax column C18 (4.6 x 150 mm, 5 µm) was utilized. Mobility Phase  with buffer : One mL of 65 formic acid :35 acetonitrile was poured into a column at a time. 0.1% Formic acid buffer was utilized in this procedure. A constant  temperature of 30°C was maintained. The ideal wavelength  was chosen as 220 nm.  Retention time of VBM and MPM were found to be 3.953, 2.364 minutes .  MPM and VBM  have respective RSD of 1.3 and 0.6. The recovery rates were 100.31%  and 100.30% with MPM and VBM, respectively. LOD and LOQ values for MPM and VBM were 0.53, 1.62, and 0.52, 1.57, respectively, according to the regression models. The regression formula for MPM was  y = 21101x + 10155,and for VBM was y = 21037x + 22037. The method was created in a simple and economical way, and it may be applied in many industries for regular quality control testing because the retention and run times were reduced.

Meropenem; pharmaceutical dosage; RP-HPLC; Vaborbactam

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