Abstract

Kamaraj Mani¹, Amit Kumar¹, Prakash Deep¹Monika Kaurav²and Roma Ghai^3*

Selected medicinal plants possess many phytochemicals that have excellent antioxidant and anti-cell proliferation potential. The banana stem extract (BSE) is also one among them which have many therapeutic values. The objective of the current experiment was to identify and confirm anti-cell proliferation activity using suitable validated in-vitro experiments. Banana stem extract was prepared by traditional extraction method. The presence of various classes of phytochemicals were confirmed using qualitative phytochemical screening tests using a standard protocol. BSE was subjected to cell viability assay for cell proliferation or cell viability using selected five organ types of human cancer cell lines. Suitable chemotherapeutic compounds were used as a reference in the above experiment. The presence of various classes of phytochemicals such as glycoside, tannin, saponin, alkaloids etc were confirmed by reaction test. Cell viability test showed favorable activity with certain types of human cancer cells. Promising inhibitory activity was seen in breast, Colon, Brain, Prostate, and lung cancer. The maximal activity was found at different concentrations in each cancer type. It is also important to note that the activity noticed was in dose-dependent manner and hence we could calculate the IC50 value. The ability inhibition of cell proliferation was encouraging with differential IC50 values. The activity could be due to the presence of various phytochemicals such as alkaloids, tannins, and glycosides in the BSE. Collectively, it can be concluded that the traditional preparation of BSE has significant anti-cancer potential in in-vitro methods. However, the same can be further explored in a suitable novel animal efficacy model with a multiparametric readout to substantiate the claim.

Breast cancer; Colon carcinoma; Glioblastoma cell line; Lung Cancer; MTT; Prostrate Cancer

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