Inhibition of Urease Catalysed Hydrolysis of Urea by Some Nitrophenols

Dilip B. Patil* and Rajendra V. Tijare

¹Department of Chemistry, Government "Science College, Gadchiroli * 442 605 India

²Department of Zoology, Government Science College, Gadchiroli - 442 605 India

ABSTRACT:

The Inhibition of urease catalysed hydrolysis of urea by phenol, o-,m-, p-Nitrophenol, 2,4-Dinitrophenol and 2,4,6-Trinitrophenol has been carried out on the basis of kinetic measurement of urea hydrolysis. The concentration of urea was varied in the range 1.3 x 103 M............. 3.33 x 10-3 M and that of inhibitors was maintained 2.7 x 10-3 M. The apparent Michaelis constants K and dissociation constants of enzyme-inhibitor complex, were determined at pH 6.70 and temperature 37°C. All the compound shows competitive type of inhibition.

KEYWORDS:

p-Nitrophenol; Hydrolysis; Michaelis; Inhibition

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Copy the following to cite this article: Patil D. B, Tijare R. V. Inhibition of Urease Catalysed Hydrolysis of Urea by Some Nitrophenols. Orient J Chem 1999;15(2).

Copy the following to cite this URL: Patil D. B, Tijare R. V. Inhibition of Urease Catalysed Hydrolysis of Urea by Some Nitrophenols. Orient J Chem 1999;15(2). Available from:http://www.orientjchem.org/?p=37114