Quantitative Analysis of Gemcitibine in Commercial Dosage Forms by Uv-Visible Spectrophotometry

M. Srinvasa Rao¹, S.V.M.Vardhan², D. Ravikumar¹ and C. Rambabu

¹Department of Chemistry, Acharya Nagarjuna University Nuzvid Campus, Nuzvid, (India).

²Department of Biochemistry, Acharya Nagarjuna University Nuzvid Campus, Nuzvid, (India).

ABSTRACT:

Two simple sensitive selective accurate and economical spectrophotometric methods A and B for the determination of Gemcitibine in bulk drug and pharmaceutical formulations (tablets) have been described in the present work. These methods are based on the formation of purple color and red- violet colored chromogens obtained when the drug was diazotized with nitrous acid followed by coupling with phloroglucinol and resorcinol, exhibiting lmax at 525 and 610 nm. These methods obey Beer’s law in the concentration ranges of 2-10 and 4-20 μg/mL.

KEYWORDS:

Gemcitibine; chromogen; spectrophotometry

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Copy the following to cite this article: Rao M. S, Vardhan S. V. M, Ravikumar D, Rambabu C. Quantitative Analysis of Gemcitibine in Commercial Dosage Forms by Uv-Visible Spectrophotometry. Orient J Chem 2008;24(3).

Copy the following to cite this URL: Rao M. S, Vardhan S. V. M, Ravikumar D, Rambabu C. Quantitative Analysis of Gemcitibine in Commercial Dosage Forms by Uv-Visible Spectrophotometry. Orient J Chem 2008;24(3). Available from: http://www.orientjchem.org/?p=25054