Application of Ninhydrin and Ascorbic Acid for The Determination of Cefpodoxime Proxetil in Pharmaceutical Formulations

A.V. Subbayamma¹* and C. Rambabu²

¹Department of Chemistry, St. Theresa’s College for Women, Eluru India.

²Department of Chemistry, Acharya Nagarjuna University P.G. Center, Nuzvid, Krishna India.

ABSTRACT:

A simple and sensitive spectrophotometric method by exploiting the importance of the condensation reaction of Ninhydrin1 indane-1,2,3- trione hydrate and Ascorbic acid2-4 for the assay of Cefpodoxime proxetil has been described (CEFP), in bulk form and dosage forms. The ninhydrin reaction is one of the most useful reactions of a-amino acids. Ninhydrin along with a reducing agent has been used as a chromogenic reagent. In the present method, the drug CEFP which possesses -NH2, when heated with ninhydrin in the presence of ascorbic acid afforded a blue violet color product. The absorption maximum was exhibited at 590nm. Regression analysis of Beer’s law plots showed good correlation in the concentration range 10-60 μg/ml.

KEYWORDS:

Cefpodoxime proxetil; Ninhydrin; Ascorbic acid; Spectrophotometry

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Copy the following to cite this article: Subbayamma A. V, Rambabu C. Application of Ninhydrin and Ascorbic Acid for The Determination of Cefpodoxime Proxetil in Pharmaceutical Formulations. Orient J Chem 2008;24(2).

Copy the following to cite this URL: Subbayamma A. V, Rambabu C. Application of Ninhydrin and Ascorbic Acid for The Determination of Cefpodoxime Proxetil in Pharmaceutical Formulations. Orient J Chem 2008;24(2). Available from: http://www.orientjchem.org/?p=24374.